Microscopes
Magnify & resolve images
 
Resolving Power
 
Resolution of a microscope is the smallest distance between 2 points that can be seen using a microscope
 
This is a measure of the clarity of the image
 
A microscope with a high resolving power will allow 2 small objects which are close together to be seen as 2 distinct objects 
The resolving power of a microscope is ultimately limited by the wavelength of light (400-600nm for visible light). To improve the resolving power a shorter
wavelength of light is needed.
 
Magnification: increase of an object’s apparent size
 
Light microscope magnify ~1500 times with a resolution of 0.2 µM
 
Electron microscope magnify ~200,000 
times with a resolution of 2 nM

 

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